Methodology for determining the sum of phenolic compounds in the mushroom Climacodon septentrionalis (Fr.) P. Karst.

Introduction. The parasitic fungus Climacodon septentrionalis (Fr.) P. Karst. has been little studied at the moment, but it is known to have antioxidant activity, which can be used in medicine. Only one medicinal product of fungal origin and a number of biologically active food supplements are registered in the Republic of Belarus. There is no information on the optimal methods and techniques for obtaining an extract from raw materials of fungal origin with the maximum possible content of phenolic compounds, as well as methods for their determination.

Alm. To develop a method for determining the content of phenolic compounds in the fungus Climacodon septentrionalis.

Materials and methods. The object of the study was the fruiting bodies of the northern Climacodon mushroom (C. septentrionalis), grown in natural conditions and stored at a temperature of –18 °C. The following reagents were used in the study: Folin – Chiocalteu reagent (LLC "Analit Komplekt", Russia), anhydrous sodium car-bonate (reagent grade, LLC "JSC REAKHIM", Russia), technical ethyl alcohol Extra-M 96.41 %, purified water; gallic acid standard (98 %, Acros Organics BVBA, Belgium); equipment: Explorer EX125D analytical scales, SOLAR PB 2201 spectrophotometer, Good-helper ES-10P10 electric hotplate, WB-12 water bath, AE-25 electric water dis-tiller.

Results and discussion. The optimal method for extracting phenolic compounds from northern Climacodon is extraction with water at a temperature of 100 °С and a raw material : extractant ratio of 1 : 20 for 150 minutes once, draining the liquid into a separate closed container, leaving it to cool at room temperature, filtering through a cotton swab, bringing it to the original volume with water if necessary. When determining the amount of phenolic compounds in terms of gallic acid, it is recommended to use 0.10 ml of the extract, 0.45 ml of Folin – Ciocalteu reagent, stirring, adding 5.25 ml of 10 % sodium carbonate solution, stirring, reaction time – 16 minutes, measuring the optical density at a wavelength of 760 nm.

Conclusion. The concentration of the sum of phenolic compounds in terms of gallic acid in the obtained extract is 202 ± 5 μg/ml or the mass fraction when converted to the wet weight of C. septentrionalis is 0.41 ± 0.01 %.

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