Optimization of the extraction of flavonoids and hydroxycinnamic acids from the genus Lamium plants by solvent

Introduction. Plants of the genus Lamium possess antioxidant, hemostatic, antispasmodic, anti-inflammatory, and other properties. Their properties are due to the content of various biologically active substances (BAS), namely flavonoids and hydroxycinnamic acids. Therefore, it is advisable to obtain extracts with increased levels of these groups of BAS.

Aim. To optimize the extraction of flavonoids and hydroxycinnamic acids from the genus Lamium plants.

Materials and methods. During the study Lamii albi herba and Lamii purpurei herba, collected in the vicinity of Minsk in May 2024, Lamii maculati herba and Lamii galeobdoli herba, collected by the V. F. Kuprevich Institute of Experimental Botany of the National Academy of Sciences of Belarus in the Volkovysk region in May 2024, were used. Quantitative determination was carried out by spectrophotometric method by reaction with aluminum chloride for flavonoids and by reaction with Arnov reagent for hydroxycinnamic acids.

Results and discussion. The study optimized extraction of flavonoids and hydroxycinnamic acids from the genus Lamium plants. It was determined that ethanol 100 % was the most effective extractant for flavonoid extraction, while propanol-2 40 % was the most effective for hydroxycinnamic acids extraction.

Conclusion. The most effective solvents and their concentrations for extraction of flavonoids and hydroxycinnamic acids were established. The results of this study will enable the optimal extraction of beneficial components from the genus Lamium plants, which will further support the development of medicinal products using these herbal raw materials.

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